Category
Antibody
Concentration
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Form
Antigen affinity purified
Conjugation
Unconjugated
Clone
Polyclonal antibody
Recognised antigen
Neurofilament (Light)
Host animal
Goat (Capra aegagrus hircus)
Clonality
Polyclonal (goat origin)
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat, Dog, Cow, Pig; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Tested applications
WB, ELISA (peptide)
Recommended dilutions
Western blot: 0.5-2ug/ml,ELISA (peptide) LOD: 1:4000
Notes
Optimal dilution of the NEFL antibody should be determined by the researcher.
Intented use
This NEFL antibodyis to be used only for research purposes and not for diagnostics..
Uniprot #
P07196
Purity
Antigen affinity
Description
Neurofilaments (NF) are the 10 nanometer or intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and are believed to function primarily to provide structural support for the axon and to regulate axon diameter. [Wiki]
Immunogen
Amino acids EKQLQELEDKQNAD were used as the immunogen for this NEFL antibody.
Storage
Aliquot and store the NEFL antibody at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps