Category
Antibody
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Form
Antigen affinity purified
Conjugation
Unconjugated
Clone
Polyclonal antibody
Recognised antigen
Alpha Internexin / Neurofilament-66
Host animal
Rabbit (Oryctolagus cuniculus)
Clonality
Polyclonal (rabbit origin)
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Tested applications
WB, IHC-P
Recommended dilutions
Western blot: 0.5-1ug/ml,IHC (FFPE): 1-2ug/ml
Added buffer
Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2.5% BSA and 0.025% sodium azide
Intented use
This Alpha Internexin antibodyis to be used only for research purposes and not for diagnostics..
Uniprot #
Q16352
Purity
Antigen affinity
Description
Alpha-Internexin (INA; also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. The protein was originally purified from rat optic nerve and spinal cord. And the protein copurifies with other neurofilament subunits, as it was originally discovered, however in some mature neurons it can be the only neurofilament expressed. The protein is present in developing neuroblasts and in the Central Nervous System of adults. Meanwhile, the protein is a major component of the intermediate filament network in small interneurons and cerebellar granule cells, where it is present in the parallel fibers.
Immunogen
Amino acids A71-R161 were used as the immunogen for the Alpha Internexin antibody.
Storage
Prior to reconstitution, store at 4oC. After reconstitution, the Alpha Internexin antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Additional description
The Anti- Alpha Internexin / Neurofilament-66 is a α- or alpha protein sometimes glycoprotein present in blood.
Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps