Category
Antibody
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Form
Antigen affinity purified
Conjugation
Unconjugated
Clone
Polyclonal antibody
Recognised antigen
CNTF / Ciliary Neurotrophic Factor
Host animal
Rabbit (Oryctolagus cuniculus)
Clonality
Polyclonal (rabbit origin)
Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Tested applications
WB, ELISA
Recommended dilutions
Western blot: 0.1-0.5ug/ml,ELISA : 0.1-0.5ug/ml
Notes
Optimal dilution of the CNTF antibody should be determined by the researcher.
Intented use
This CNTF antibodyis to be used only for research purposes and not for diagnostics..
Uniprot #
P26441
Purity
Antigen affinity
Description
Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid (aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding human CNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. It is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
Immunogen
Amino acids 12-200 of human CNTF were used as the immunogen for the CNTF antibody.
Storage
After reconstitution, the CNTF antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Additional description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
French translation
anticorps