Category
Antibody
Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
Form
Antigen affinity purified
Conjugation
Unconjugated
Clone
Polyclonal antibody
Recognised antigen
Neuron Specific Enolase (NSE)
Host animal
Rabbit (Oryctolagus cuniculus)
Clonality
Polyclonal (rabbit origin)
Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Tested applications
WB, IHC, ELISA
Recommended dilutions
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Notes
The stated application concentrations are suggested starting amounts. Titration of the Neuron Specific Enolase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Intented use
This Neuron Specific Enolase antibodyis to be used only for research purposes and not for diagnostics..
Uniprot #
P09104
Purity
Antigen affinity
Description
NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha to gamma enolase occurs in neural tissue during development in rats and primates. [Wiki]
Immunogen
A portion of amino acids 213-239 from human ENO2 was used as the immunogen for this Neuron Specific Enolase antibody.
Storage
Store at 4oC for up to one month. For long term, aliquot the Neuron Specific Enolase antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps