Long name
Mouse Monoclonal Antibody against Human Neurofilament heavy protein, Clone: NF-05
Antigen
Neurofilament heavy protein
Species reactivity
Human (Homo sapiens), Porcine, Rat
Negative species reactivity
Mouse
Clonality
Monoclonal Antibody (Mab)
Clone
NF-05
Applications
WB, IHC(F), ELISA
Immunogen
Pig brain neurofilament protein-enriched fraction after depolymerization of microtubules
Format/conjugation
purified; unconjugated
Specificity
The antibody NF-05 recognizes a nonphosphorylated epitope of neurofilament heavy protein (NF-H), a 210 kDa intracellular structural protein of Intermediate Filament Proteins family. NF-H is mainly expressed in the central and peripheral nervous system and reproductive system and is biochemically very stable. _x000D_
Research area
Cytoskeleton: Intermediate Filaments (Veterinary), Cytoskeleton: Intermediate Filaments (Rodent), Neurobiology (Veterinary), Neurobiology (Rodent), Neurobiology (Human), Cytoskeleton: Intermediate Filaments (Human), Cytoskeleton: Intermediate Filaments
Concentration
1 mg/ml
Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Shipping and storage
Mouse Monoclonal Antibody against Human Neurofilament heavy protein, Clone: NF-05 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
Background
Neurofilaments (NFs) are a type of intermediate filaments (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights – neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.
Purity
> 95% (by SDS-PAGE)
Purification method
Purified by precipitation and chromatography
References
*Porchet R, Probst A, Draberova E, Draber P, Riederer IM, Riederer BM: Differential subcellular localization of phosphorylated neurofilament and tau proteins in degenerating neurons of the human entorhinal cortex._x000D_ Neuroreport. 2003 May 23;14(7):929-33. _x000D_
Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
French translation
anticorps