Clone
S364-51
Immunogen
Fusion protein amino acids 30-845 (extracellular domain) of mouse NrCAM. Rat: 96% identity (795/822 amino acids identical). Human: 91% identity (753/822 amino acids identical) ~50% identity with Neurofascin.
Antibody's full description
Mouse Anti-Mouse NrCAM Monoclonal IgG2a Antibody, Clone: S364-51: ATTO 565
Antibody's category
Monoclonal Antibodies
Antibody's other name
Neuronal cell adhesion molecule Antibody, Bravo Antibody, hBravo Antibody, KIAA0343 Antibody, MGC138845 Antibody, MGC138846 Antibody, Neuronal surface protein Bravo Antibody, Ng CAM related Antibody, Ng-CAM-related Antibody, NgCAM related cell adhesion molecule Antibody, NgCAM-related cell adhesion molecule Antibody, Nr CAM Antibody, Nr-CAM Antibody
Raised in
Mouse
Antibody's target
NrCAM
Primary research fields
Neuroscience, Cell Structure, Membrane Markers
Brandname
None
Antibodies' applications
WB, ICC/IF
Antibody's reactivity
Human, Mouse, Rat
Antibody's dilutions
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purity
Protein G Purified
Antibody buffer for storage
PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Antibody's concentration
1 mg/ml
Antibody's specificity
Detects ~160kDa.
Storage recommendations
-20ºC
Shipping recommendations
Blue Ice or 4ºC
Antibody certificate of analysis
1 µg/ml of SMC-462 was sufficient for detection of NrCAM in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody in cell
Cell Membrane
Tissue specificity
See included datasheet or contact our support service
Scientific context
Neuronal cell adhesion molecule (NrCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NrCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NrCAM expression may play a role in the severity of certain types of tumors. NrCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NrCAM maps to chromosome 7q31.1-q31.2.
Released date
29-Oct-2013
Tested applications
To be tested
Tested reactivity
To be tested
NCBI number
NP_001139503.1
Gene number
319504
Protein number
Q810U4
Antibody's datasheet
Contact our support service
Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NrCAM Monoclonal Antibody, Clone S364-51 (SMC-462). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NrCAM Monoclonal Antibody (SMC-462) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NrCAM Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51 (SMC-462). Load: 10 µg. Primary Antibody: Mouse Anti-NrCam Monoclonal Antibody (SMC-462) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~160 kDa. Mouse Anti-NrCAM Antibody [S364-51] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-462) Mouse Anti-NrCAM Antibody [S364-51] used in Western Blot (WB) on Rat Brain Membrane (SMC-462)
Warning information
Non-hazardous
Country of production
Canada
Total weight (kg)
1.4
Net weight (g)
0.1
Stock availability
In Stock
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Additional isotype
IgG2a
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus