Dilutions
ICC 1:1000; IHC 1:1000; WB 1:10,000
Immunogen
Bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. The form of NF-H isolated is the heavily phosphorylated axonal variant, and the antibody generated recognizes predominantly axonal neurofilaments.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Storage Location
Frozen (Polar Packs)
Entrez
4744
UniProt
P12036
RRID
NA
Applications
ICC, WB, IHC
Types
Rabbit IgG
Format A
Whole serum
Format B
liquid