Disaggregated paired helical filaments were used as an immunogen. Insoluble NFT which were extracted in boiling sodium dodecyl sulfate, purified by sucrose gradient centrifugation and disaggregated by sonication were used as an immunogen for the preparation of this antibody.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.