Long name
RNF131 Polyclonal Antibody, Biotin Conjugated
Category
Conjugated Primary Antibodies
Conjugation
Biotin
Host Organism
Rabbit (Oryctolagus cuniculus)
Target Antigen
RNF131
Specificity
This is a highly specific antibody against RNF131.
Modification
Unmodified
Modification site
None
Clonality
Polyclonal
Clone
Polyclonal antibody
Concentration
1ug per 1ul
Source
KLH conjugated synthetic peptide derived from human PJA2/RNF131
Gene ID number
9867
Tested applications
WB, IHC-P
Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500)
Crossreactivity
Human, Mouse, Rat
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Antigen background
PJA2, also known as E3 ubiquitin-protein ligase praja-2, RNF131 (ring finger protein 131) or Neurodap1, is a 708 amino acid protein that contains one ring-type zinc finger and exists as two alternatively spliced isoforms. Significantly conserved in chimpanzee, dog, cow, mouse, rat, chicken and zebrafish, PJA2 shares 52% identity with PJA1, which is involved in protein ubiquitination in brain and may play a role in X-linked mental retardation. Encoded by a gene that maps to human chromosome 5q21.3, PJA2 localizes to both endoplasmic reticulum and Golgi apparatus membranes. Participating in E2-dependent, E3 ubiquitin-protein ligase activity, PJA2 binds to a variety of E2s and interacts with ubiquitin-conjugating enzymes, such as UBE2D2, in vitro.
Purification method
This antibody was purified via Protein A.
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Excitation Emission
N/A
Synonyms
E3 ubiquitin protein ligase Praja2; E3 ubiquitin-protein ligase Praja-2; KIAA0438; Neurodap 1; Neurodap1; PJA 2; PJA2; PJA2_HUMAN; Praja 2; Praja 2 RING H2 mot containing; praja ring finger 2; Praja ring finger 2 E3 ubiquitin protein ligase; Praja2; RING finger protein 131; RNF 131; RNF131.
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Additional conjugation
Biotinylated
French translation
anticorps