Neuro D2 Antibody, FITC Conjugated

Basic informations

  • Size: 0.1ml
  • Catalog number: bs-11918R-FITC
  • Price: 333.00EUR
Neuro D2 Antibody, FITC Conjugated

Long name

Neuro D2 Polyclonal Antibody, FITC Conjugated

Category

Conjugated Primary Antibodies

Conjugation

FITC

Host Organism

Rabbit (Oryctolagus cuniculus)

Target Antigen

Neuro D2

Specificity

This is a highly specific antibody against Neuro D2.

Modification

Unmodified

Modification site

None

Clonality

Polyclonal

Clone

Polyclonal antibody

Concentration

1ug per 1ul

Source

KLH conjugated synthetic peptide derived from human Neuro D2

Tested applications

IF(IHC-P)

Recommended dilutions

IF(IHC-P)(1:50-200)

Crossreactivity

Human, Mouse, Rat

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Antigen background

Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes, both of which work together to activate DNA transcription. Class A proteins include the ubiquitously expressed E-box binding factors, namely E2A, ITF-2 and HEB, while class B proteins, such as MyoD, myogenin and Neuro D (BETA2), are transiently expressed and exhibit a much more limited tissue distribution. Working in opposition to these positively acting factors are a specialized group of basic helix-loop-helix (bHLH) transcription factors that function as dominant negative regulators and are involved in cell lineage determination and differentiation. Neuro D2 (neurogenic differentiation 2), also known as NDRF, NEUROD2 or bHLHa1, is a 382 amino acid nuclear protein that contains one bHLH domain and functions to induce neurogenic differentiation, playing an important role in the maintenance and determination of cell fate.

Purification method

This antibody was purified via Protein A.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Excitation Emission

494nm/518nm

Synonyms

bHLHa1; Class A basic helix-loop-helix protein 1; class A basic helix loop helix protein 1; NDF2_HUMAN; NDR2; NDRF; Neuro-D2; NeuroD-related factor; NeuroD2; Neurogenic dferentiation factor 2; NDF2_HUMAN; neuroD related factor; NeuroD2; neurogenic basic helix loop helix protein; neurogenic dferentiation 2; Neurogenic dferentiation factor 2; neuronal dferentiation 2.

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.

Additional conjugation

Anti-FITC Antibody

French translation

anticorps