Detection Type
Colormetric
Target Species
Rat
Target Synonym
TRKB, trk-B, GP145-TrkB, TrkB receptor, TrkB tyrosine kinase, BDNF/NT-3 growth factors receptor
Test principle
This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NTRK2 . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NTRK2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NTRK2 , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NTRK2 . You can calculate the concentration of Rat NTRK2 in the samples by comparing the RLU value of the samples to the standard curve.
Sensitivity
9.38pg/mL
Detection Range
15.63~1000pg/mL
Target Name
NTRK2
UNIProt ID
NA
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Rat NTRK2 (Neurotrophic Tyrosine Kinase Receptor Type 2) CLIA Kit
Type
Sandwich
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus
Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.