Detection Type
Colormetric
Target Species
Human
Target Synonym
NK-B, TAC3, NKNB, Tachykinin 3, Neurokinin Beta
Test principle
This EIA kit uses the Competitive-EIA principle. The micro EIA plate provided in this kit has been pre-coated with Human NKB. During the reaction, Human NKB in the sample or standard competes with a fixed amount of Human NKB on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human NKB. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human NKB in the samples is then determined by comparing the OD of the samples to the standard curve.
Sensitivity
9.38pg/mL
Detection Range
15.63~1000pg/mL
Target Name
NKB
UNIProt ID
NA
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Human NKB(Neurokinin B) EIA Kit
Type
Competitive
Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.