Detection Type
Colormetric
Target Species
Porcine
Target Synonym
NA
Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Porcine NSE. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine NSE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine NSE. You can calculate the concentration of Porcine NSE in the samples by comparing the OD of the samples to the standard curve.
Sensitivity
0.94ng/mL
Detection Range
1.56~100ng/mL
Target Name
NSE
UNIProt ID
NA
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Porcine NSE(Neuron Specific Enolase) EIA Kit
Type
Sandwich
Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay