Detection Type
Colormetric
Target Species
Rat
Target Synonym
NP-Y, PYY4
Test principle
This EIA kit uses the Competitive-EIA principle. The micro EIA plate provided in this kit has been pre-coated with Rat NPY. During the reaction, Rat NPY in the sample or standard competes with a fixed amount of Rat NPY on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat NPY. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat NPY in the samples is then determined by comparing the OD of the samples to the standard curve.
Sensitivity
18.75pg/mL
Detection Range
31.25~2000pg/mL
Target Name
NPY
UNIProt ID
P07808
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Rat NPY(Neuropeptide Y) EIA Kit
Type
Competitive
Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus