Synonym
Cntfr
Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat CTNFR at 10 μg/ml (100 μl/well) can bind biotinylated human CNTF with a linear ranger of 1.28-160 ng/ml.
Sequence
Met 1-Pro 346
Fusion tag
C-His
Accession
Q08406-1
ExpressedHost
Baculovirus-Insect Cells
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Purity
> 97 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Mol Mass
39.6 kDa
AP Mol Mass
48 KDa
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0
Background
Ciliary neurotrophic factor(CNTF) is a member of the cytokine family. It is a polypeptide hormone that have functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It's actions appear to be restricted to the nervous system. Ciliary neurotrophic factor(CNTF) has biological effects through the activation of a multi-subunit receptor complex, consisting of an extracelluar CNTF binding subunit(CNTFα) and two transmembrane signal transduction proteins: glycoprotein gp130 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF is also a survival factor for neurons of the peripheral sensory sympathetic, and ciliary ganglia. It has been reported that CNTF could be an agent that has therapeutic potential and possibly induces differentiation of large multipolar ganglionic phenotype in a subset of progenitors.
Description
The Recombinant CNTFR / CNTFR-alpha Protein (His tag) is a α- or alpha protein sometimes glycoprotein present in blood.
Conjugation
histidine
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus
Source
Recombinants or rec. proteins
Group
recombinants