Synonym
ErbB2;Neu;p185erbB2
Activity
1.Measured by its binding ability in a functional ELISA. 2.Immobilized rat ErbB2 at 10μg/mL (100μL/well) can bind herceptin, the EC50 of herceptin is 30-90ng/mL.
Sequence
Met 4-Thr 656
Fusion tag
NA
Accession
AAH61863.1
ExpressedHost
HEK293 Cells
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Purity
> 92 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Mol Mass
70.6 kDa
AP Mol Mass
95-105 KDa
Formulation
Lyophilized from sterile PBS, pH 7.4
Background
Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus
Source
Recombinants or rec. proteins
Group
recombinants