Synonym
Neurotrophin-3; NT-3; HDNF; Nerve Growth Factor 2; NGF-2; Neurotrophic Factor; NTF3
Activity
Measured by its ability to bind Human NTRK2 in functional ELISA.The ED50 for this effect is less than 10 ug/ml.
Sequence
Tyr139-Thr257
Fusion tag
NA
Accession
P20783
Expressed Host
E.coli
Shipping
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Purity
＞95% as determined by reducing SDS-PAGE.
Endotoxin
＜1.0 EU per µg as determined by LAL test.
Stability and Storage
Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
Mol Mass
13.6 kDa
AP Mol Mass
14 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.
Reconstitution
Please refer to it for detailed information.
Background
Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors and is structurally related to β-NGF, BDNF and NT-4. The NT3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT3.The amino acid sequences of mature human, murine and rat NT-3 are identical. NT-3 selectively promotes the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Source
Recombinants or rec. proteins
Group
recombinants