Estimated Turnaround Time
6-11 business days
Target Name
NA
Target's alterntive name
NPY
Species Reactivity
Mouse
Sample Type
Serum, Plasma, Biological Fluids
Detection Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL
Precision
NA
Detection Method
Colorimetric
Assay Time
2.5 hours
Application
For research use only. Not for diagnostic procedures.
Assay Type
Competitive
Shipping Conditions
Ice packs
Storage Temperature
Short term: 4°C; Long term: see manual.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Shelf life
Use Mouse NPY (Neuropeptide Y) ELISA Kit before 6 months
Specificity
NA
Cross Activity
No significant cross-reactivity or interference was observed.
Test Principle
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Mouse NPY. During the reaction, Mouse NPY in the sample or standard competes with a fixed amount of Mouse NPY on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse NPY. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse NPY in the samples is then determined by comparing the OD of the samples to the standard curve.
Stability
NA
Quality Systems
The kit is manufactured at ISO 9001 certified facilities.
Research Area
Cancer, Cardiovascular, Metabolism, Neuroscience
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus