6-11 business days
NA
NT
Human
Serum, Plasma, Biological Fluids
78.13-5000 pg/mL
46.88 pg/mL
NA
Colorimetric
2.5 hours
For research use only. Not for diagnostic procedures.
Competitive
Ice packs
Short term: 4°C; Long term: see manual.
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Use Human NT (Neurotensin) ELISA Kit before 6 months
NA
No significant cross-reactivity or interference was observed.
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human NT. During the reaction, Human NT in the sample or standard competes with a fixed amount of Human NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human NT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human NT in the samples is then determined by comparing the OD of the samples to the standard curve.
NA
The kit is manufactured at ISO 9001 certified facilities.
Metabolism, Neuroscience, Signal transduction
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED