Estimated Turnaround Time
6-11 Business days
Target Name
Glial Cell Line Derived Neurotrophic Factor
Target's alterntive name
ATF1, ATF2, HFB1-GDNF, Astrocyte-Derived Trophic Factor, Glial Derived Neurotrophic Factor
Species Reactivity
Rattus norvegicus (Rat)
Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
78-5000 pg/mL
Sensitivity
32 pg/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
Detection Method
Colorimetric
Assay Time
3 hours
Application
For research use only. Not for diagnostic procedures.
Assay Type
Double-antibody Sandwich
Shipping Conditions
Ice packs
Storage Temperature
Short term: 4°C; Long term: see manual.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Shelf life
Use Rat Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit before 8 months
Specificity
This assay has high sensitivity and excellent specificity for detection of Glial Cell Line Derived Neurotrophic Factor
Cross Activity
No significant cross-reactivity or interference between Glial Cell Line Derived Neurotrophic Factor and analogues was observed.
Test Principle
No significant cross-reactivity or interference between Glial Cell Line Derived Neurotrophic Factor and analogues was observed.
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area
Cytokine, Apoptosis, Neuro science
Tissue
cell
Description
For cells, cell lines and tissues in culture till half confluency.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus