Mouse Monoclonal IgG1 Anti-N-CAM, Neuronal Cell Adhesion Molecule [RNL-1]

Availability

Please contact us to check the availability

Immunogen

NCI-H82 cell preparation

Specificity

Extracellular domain of the N-CAM molecule (200, 100 and 25-27 kDa proteins of NCI-H82 SCLC cell line in western blot)

Species reactivity

Human

Product presentation

Cell culture supernatant containing 0.09% sodium azide as preservative

Product category

Primary Antibody

Antibody's raised in

Mouse

Antibody's isotype

IgG1

Antibody's clone

RNL-1

Product is supplied as

liquid concentrate

Recommended concentration for use

1:10-1:25

Protein concentration

N/A

Positive control

Pancreas islets or adrenal medulla

Tested applications

IHC(C), suitability for paraffin sections not established. Glutaraldehyde fixation for EM has been used successfully (Broers et al. 1991).

Recommended secondary reagent

We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage method

Store at 2-8°C

UniProt number

Go to UniProt/SwissProt

Litterature

1. Broers V.J.L., Mijnheere E.P., Rot M.K., Schaart G. Sijlmans A., Boerman O.C., and Raaemaekers F.C.S. (1991) Novel antigens charakteristic of neuroendocrine malignancies. Cancer 67,3; 619-633. 2. Boerman O.C., Mijnheere E.P., Broers J.L.V., Vooijs G.P., and Ramaekers F.C.S. (1991) Biodistribution of a monoclonal antibody (RNL-1) against the neural cell adhesion molecule (NCAM) in athymic mice bearing human small-cell lung-cancer xenografts. Int. J. Cancer 48; 457-462. 3. Stahel R.A., Gilks W.R., Schenker Th., and Gerardy-Schahn R. (1995) NK3.1 Third International Workshop on Lung Tumour and Differentiation Antigens: summary and report on NCAM studies. In: Leucocyte Typing V. Schlossman S.F. et al. eds. Oxford University Press, Oxford, N.Y. Tokyo.

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Tissue

cell

Description

cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Research.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus