Mouse Monoclonal IgG1 Anti-Neurofilaments 70/200 kDa [2F11]

Availability

Please contact us to check the availability

Immunogen

Purified neurofilament proteins from human brain

Specificity

Neurofilaments 70 and 200 kDa

Species reactivity

Human

Product presentation

Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.

Product category

Primary Antibody

Antibody's raised in

Mouse

Antibody's isotype

IgG1

Antibody's clone

2F11

Product is supplied as

liquid concentrate

Recommended concentration for use

1:10 - 1:20

Protein concentration

N/A

Positive control

Nerve tissue

Pretreatment

No protease pre-treatment required! Blocking of endogenous biotin and alkaline phosphatase is recommended, if avidin/biotin systems are used.

Tested applications

IHC(P)

Recommended secondary reagent

We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

Storage method

Store at 2-8°C

UniProt number

Go to UniProt/SwissProt

Litterature

1. Osborn M., Altmannsberger M., Shaw G., Schauer A., Weber K. (1982) Various sympathetic derived human tumors differ in neurofilament expression. Use in Diagnosis of neuroblastoma, ganglioneuroblastoma and pheochromocytoma. Virchows Arch. Cell Pathol. 40; 141-156. 2. Alfonsi F., Darmon M., Forest N., and Paulin D. (1983) Intermediate sized filaments as markers of neuronal differentiation in the role of cell interaction in early neurogenesis. In: Plenum Publishing Corporation A.M. Duprat, A. Kato, and M. Weber eds. pp 157-176. 3. Osborn M., and Weber K. (1983) Biology of disease tumor diagnosis by intermediate filament typing. Lab. Invest. 48; 372-394. 4. Klück P., et al. (1984) Hirschsprung disease studied with monoclonal anti neurofilament antibodies on tissue sections. The Lancet, March 24, 652-653. 5.. Osborn M., Dirk T., Käser H., Weber K., and Altmannsberger M. (1986) Immunohistochemical localization of neurofilaments and neuron-specific enolase in 29 cases of neuroblastoma. Am. J. Pathol. 122; 433-442.

Tips

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The kilo Daltons subunit weight of Bioprime compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Latin name

Mus musculus