Antibody
In 1X PBS, pH 7.4, with 0.09% sodium azide
Antigen affinity purified
Unconjugated
Polyclonal antibody
Neuron Specific Enolase (NSE)
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
WB, IHC, ELISA
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Titration of the NSE antibody may be required due to differences in protocols and secondary/substrate sensitivity.
This NSE antibodyis to be used only for research purposes and not for diagnostics..
P09104
Antigen affinity
Neuron Specific Enolase, also called Gamma-enolase, and Enolase 2 (ENO2) has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). [UniProt]
A portion of amino acids 6-32 from the human protein was used as the immunogen for this NSE antibody.
Aliquot the NSE antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
anticorps