Type
Conjugated Primary Antibody
Conjugated with
ALEXA FLUOR® 594
Host organism
Rabbit (Oryctolagus cuniculus)
Target Protein/Peptide
GPR136/Neuropsin
Specificity
This antibody reacts specifically with GPR136/Neuropsin
Modification
No modification has been applied to this antibody
Modification site
None
Clonality
Polyclonal Antibody
Clone
Polyclonal Antibodies
Concentration
1ug per 1ul
Subcellular locations
Extracellular
Antigen Source
KLH conjugated synthetic peptide derived from human G protein-coupled receptor 136
Gene ID
221391
Swiss Prot
N/A
Applications
FCM, IF(IHC-P)
Applications with corresponding dilutions
FCM(1:20-100), IF(IHC-P)(1:50-200)
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Background information
GPCR GPR136, also known as Opsin 5, is a member of the guanine nucleotide binding protein (G protein) coupled receptor superfamily. This gene belongs to the seven exon subfamily of mammalian opsin genes that includes peropsin (RRH) and retinal G protein coupled receptor (RGR). Like these other seven exon opsin genes, this gene may encode a protein with photoisomerase activity. Alternative splicing results in multiple transcript variants encoding different isoforms. ESTs for the GPR136 gene have been identified from human normal testis and B cell/lung/testis libraries..
Purification method
Purified by Protein A.
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Excitation emission
590nm/617nm
Synonyms
G protein-coupled receptor 136; G-protein coupled receptor PGR12; Gpr136; HGNC:21318; Neuropsin; OPN5; Opsin 5; PGR12; TMEM13; Transmembrane protein 13; OPN5_HUMAN; GPCR GPR136.
Also known as
GPR136/Neuropsin Polyclonal Antibody
Other name
Anti-GPR136/Neuropsin Polyclonal
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Properties
For facs or microscopy Alexa 1 conjugate.
Conjugation
Alexa Fluor,ALEXA FLUOR® 594
Conjugated
Alexa conjugate 1
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.