Type
Conjugated Primary Antibody
Conjugated with
ALEXA FLUOR® 594
Host organism
Rabbit (Oryctolagus cuniculus)
Target Protein/Peptide
Neurotrimin/HNT
Specificity
This antibody reacts specifically with Neurotrimin/HNT
Modification
No modification has been applied to this antibody
Modification site
None
Clonality
Polyclonal Antibody
Clone
Polyclonal Antibodies
Concentration
1ug per 1ul
Subcellular locations
N/A
Antigen Source
KLH conjugated synthetic peptide derived from human Neurotrimin/HNT
Gene ID
N/A
Swiss Prot
N/A
Applications
IF(IHC-P)
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Background information
Cell adhesion molecules (CAMs) influence cell growth, differentiation, embryogenesis, immune response and cancer metastasis by networking information from the extracellular matrix to the cell. The four major families of cell adhesion molecules are immunoglobulin (Ig) superfamily (calcium-independent transmembrane glycoproteins), integrins (transmembrane non-covalently linked heterodimers of Alpha and Beta subunits), calcium-dependent cadherins and divalent cation-dependent selectins. Regulation of neuronal synaptic adhesion by CAMs has proven important for learning and memory. Proper embryonic morphogenic development is also heavily dependent on the regulation of cell adhesion molecules. Neurotrimin (hNT) is a neural cell adhesion molecule localizing to the cell membrane, where it acts as a lipid-anchor. Neurotrimin belongs to the IgLON family of proteins, a member of the larger immunoglobulin superfamily.
Purification method
Purified by Protein A.
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Excitation emission
590nm/617nm
Synonyms
hNT; IgLON family member 2; IGLON2; Neurotrimin; NT antibody NTM; NTRI_HUMAN.
Also known as
Neurotrimin/HNT Polyclonal Antibody
Other name
Anti-Neurotrimin/HNT Polyclonal
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Properties
For facs or microscopy Alexa 1 conjugate.
Conjugation
Alexa Fluor,ALEXA FLUOR® 594
Conjugated
Alexa conjugate 1
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.