Clone
S110-29
Immunogen
Fusion protein amino acids 730-848 (intracellular C-terminus) of rat Neuroligin-3. Mouse: 99% identity (118/119 amino acids identical). Human: 98% identity (116/119 amino acids identical) ~60% identity with Neuroligin-1. ~40% identity with Neuroligin-2.
Antibody's full description
Mouse Anti-Rat Neuroligin 3 Monoclonal IgG1 Antibody, Clone: S110-29: ATTO 700
Antibody's category
Monoclonal Antibodies
Antibody's other name
Gliotactin homolog Antibody, Neuroligin-3 Antibody, Nlgn3 Antibody, NLGN3_HUMAN Antibody
Raised in
Mouse
Antibody's target
Neuroligin 3
Primary research fields
Neuroscience, Cell Structure, Post-Synaptic Markers
Brandname
None
Antibodies' applications
WB, IHC, ICC/IF
Antibody's reactivity
Human, Mouse, Rat
Antibody's dilutions
WB (1:1000); optimal dilutions for assays should be determined by the user.
Purity
Protein G Purified
Antibody buffer for storage
PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Antibody's concentration
1 mg/ml
Antibody's specificity
Detects ~110kDa. Does not cross-react with Neuroligin-1, -2, -4 or -4.
Storage recommendations
-20ºC
Shipping recommendations
Blue Ice or 4ºC
Antibody certificate of analysis
1 µg/ml of SMC-471 was sufficient for detection of Neuroligin 3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody in cell
Cell Membrane , Cell Junction , Synapse
Tissue specificity
See included datasheet or contact our support service
Scientific context
Neuroligins are Type I membrane proteins enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities. They have been characterized as neuronal cell surface proteins and are thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. They play a major role in the formation or maintenance of synaptic junctions. They are also thought to be involved in the specification of excitatory synapses. Neuroligins interact with beta-neurexins and this interaction is involved in the formation of functional synapses.
Released date
29-Oct-2013
Tested applications
To be tested
Tested reactivity
To be tested
NCBI number
AAA97871
Gene number
171297
Protein number
Q62889
Antibody's datasheet
Contact our support service
Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29 (SMC-471). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody (SMC-471) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction, Synapse . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Neuroligin 3 Antibody (D) Composite. Western Blot analysis of Mouse Brain Membrane showing detection of ~110 kDa Neuroligin 3 protein using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29 (SMC-471). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody (SMC-471) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa. Mouse Anti-Neuroligin 3 Antibody [S110-29] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-471) Mouse Anti-Neuroligin 3 Antibody [S110-29] used in Western Blot (WB) on Mouse Brain Membrane (SMC-471)
Warning information
Non-hazardous
Country of production
Canada
Total weight (kg)
1.4
Net weight (g)
0.1
Stock availability
In Stock
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Additional isotype
IgG1
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus,Rattus norvegicus