Clone
S98-7
Immunogen
Fusion protein amino acids 782-945 (intracellular C-terminus) of mouse Neuroligin 4. ~45% identity with Neuroligin-1, -2 and -3.
Antibody's full description
Mouse Anti-Mouse Neuroligin 4 Monoclonal IgG1 Antibody, Clone: S98-7: ATTO 633
Antibody's category
Monoclonal Antibodies
Antibody's other name
ASPGX2 Antibody, AUTSX2 Antibody, HLNX Antibody, HNLX Antibody, KIAA1260 Antibody, MGC22376 Antibody, NLGN Antibody, NLGN4 Antibody, NLGN4X Antibody, neuroligin-4 X-linked Antibody, neuroligin X Antibody, neuroligin 4 Antibody, X-linked Antibody
Raised in
Mouse
Antibody's target
Neuroligin 4
Primary research fields
Neuroscience, Cell Structure, Post-Synaptic Markers
Brandname
None
Antibodies' applications
WB, IHC, ICC/IF
Antibody's reactivity
Mouse
Antibody's dilutions
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purity
Protein G Purified
Antibody buffer for storage
PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Antibody's concentration
1 mg/ml
Antibody's specificity
Detects ~125-130kDa. Does not cross-react with other Neuroligins.
Storage recommendations
-20ºC
Shipping recommendations
Blue Ice or 4ºC
Antibody certificate of analysis
1 µg/ml of SMC-469 was sufficient for detection of Neuroligin 4 in 20 µg of mouse brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody in cell
Cell Membrane , Cell Junction
Tissue specificity
See included datasheet or contact our support service
Scientific context
This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. The encoded protein interacts with discs, large (Drosophila) homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. Two transcript variants encoding the same protein have been identified for this gene.
Released date
20-Nov-2013
Tested applications
To be tested
Tested reactivity
To be tested
NCBI number
ABS19580
Gene number
100113365
Protein number
B0F2B4
Antibody's datasheet
Contact our support service
Representative figure link
| _Antibody_S98-7_WB_Rat_Brain-Membrane_1.png
Representative figure legend
Immunohistochemistry analysis using Mouse Anti-Neuroligin 4 Monoclonal Antibody, Clone S98-7 (SMC-469). Tissue: Dentate Gyrus. Species: Mouse. Fixation: formaldehyde-fixed paraffin embedded. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody (SMC-469) at 1:100. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain at 1:1000. Courtesy of: Rachel Reith, NIH/NIMH. Western Blot analysis of Rat Brain Membrane showing detection of ~125-130 kDa Neuroligin 4 protein using Mouse Anti-Neuroligin 4 Monoclonal Antibody, Clone S98-7 (SMC-469). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody (SMC-469) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~125-130 kDa. Mouse Anti-Neuroligin 4 Antibody [S98-7] used in Immunohistochemistry (IHC) on Mouse Dentate Gyrus (SMC-469) Mouse Anti-Neuroligin 4 Antibody [S98-7] used in Western Blot (WB) on Rat Brain Membrane (SMC-469)
Warning information
Non-hazardous
Country of production
Canada
Total weight (kg)
1.4
Net weight (g)
0.1
Stock availability
In Stock
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Additional isotype
IgG1
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus