Immunogen
Synthetic peptide from the mid-protein of Human GDNF
Antibody's target
Human GDNF
Antibody's full description
Rabbit Anti-Human GDNF Polyclonal
Primary research fields
Neuroscience, Neurodegeneration, Parkinson’s Disease, Neuroscience, Neurodegeneration, ALS Disease
Antibody's category
Polyclonal Antibodies
Antibody's other name
Glial cell line-derived neurotrophic factor Antibody, Astrocyte-derived trophic factor Antibody, Astrocyte derived trophic factor 1 Antibody, Glial cell derived neurotrophic factor Antibody, ATF 1 Antibody, ATF 2 Antibody, Glial Cell Line Derived Neurotrophic Factor Antibody, ATF Antibody, Atf Antibody, HSCR3 Antibody, Astrocyte derived trophic factor Antibody, ATF2 Antibody, GDNF_HUMAN Antibody, GDNF Antibody, Glial derived neurotrophic factor Antibody, ATF1 Antibody, gdnf Antibody, HFB1 GDNF Antibody, hGDNF Antibody
Verified applications
WB, ICC/IF
Raised in
Rabbit
Antibody's reactivity
Human, Rat
Antibody's recommended dilutions for use
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody's purified from
Peptide Affinity Purified
Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
Antibody's concentration
1 mg/ml
Antibody's specificity
Detects ~24 kDa.
Storage recommendations
-20°C
Shipping recommendations
Blue Ice or 4°C
Certificate of analysis
A 1:1000 dilution of SPC-710 was sufficient for detection of GDNF in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell
Secreted
Tissue specificity
In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.
Scientific context
See included datasheet or contact our support service.
Bibliography
Refer to PubMed
Released date
25/Oct/2016
NCBI number
NP_000505.1
Gene number
2668
Protein number
P39905
PubMed number
Refer to PubMed
Tested applications
To be tested
Tested reactivity
To be tested
Antibody's datasheet
Contact our support service to receive datasheet or other technical documentation.
Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GDNF Polyclonal Antibody (SPC-710). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody (SPC-710) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Vesicles, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GDNF Antibody (D) Composite. | Western blot analysis of Human HeLa cell lysates showing detection of ~23.7 kDa GDNF protein using Rabbit Anti-GDNF Polyclonal Antibody (SPC-710). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody (SPC-710) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~23.7 kDa. Other Band(s): 50 kDa is a dimer. Rabbit Anti-GDNF Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-710) | Rabbit Anti-GDNF Antibody used in Western blot (WB) on HeLa cell lysates (SPC-710)
Warning information
Non-hazardous
Country of production
Canada
Total weight (kg)
1.4
Net weight (g)
0.1
Stock availabilit
In Stock
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name
Oryctolagus cuniculus