A synthetic peptide corresponding to a sequence at the C-terminus of human nNOS(neuronal) (1402-1434aa VTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
Storage condtions
Keep the nNOS(neuronal) Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
Tips
The nNOS(neuronal) Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
Background
Nitric oxide synthase 1 (neuronal), also known as NOS1, is an enzyme that in humans is encoded by the NOS1 gene. The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.
Related articles
1. "Entrez Gene: NOS1 Nitric oxide synthase 1 (neuronal)". 2. Shteyer, E., Edvardson, S., Wynia-Smith, S. L., Pierri, C. L., Zangen, T., Hashavya, S., Begin, M., Yaacov, B., Cinamon, Y., Koplewitz, B. Z., Vromen, A., Elpeleg, O., Smith, B. C. Truncating mutation in the nitric oxide synthase 1 gene is associated with infantile achalasia. Gastroenterology 148: 533-536, 2015. 3. Xu, W., Gorman, P., Sheer, D., Bates, G., Kishimoto, J., Lizhi, L., Emson, P. Regional localization of the gene coding for human brain nitric oxide synthase (NOS1) to 12q24.2-24.31 by fluorescent in situ hybridization. Cytogenet. Cell Genet. 64: 62-63, 1993.
