A synthetic peptide corresponding to a sequence in the middle region of human GDNF(142-158aa FRYCSGSCDAAETTYDK), different from the related mouse sequence by three amino acids and rat sequence by two amino acids.
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
Storage condtions
Keep the GDNF Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
Tips
The GDNF Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
Background
GDNF, Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene. GDNF is a small protein that potently promotes the survival of many types of neurons. GDNF is a founding member of the GDNF family of ligands(GFL). The GDNF gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization(FISH). This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET(rearranged during transfection) protooncogene.
Related articles
1. Iwashita, T., Kruger, G. M., Pardal, R., Kiel, M. J., Morrison, S. J.Hirschsprung disease is linked to defects in neural crest stem cell function.Science 301: 972-976, 2003. 2. Lin, L.-F. H., Doherty, D. H., Lile, J. D., Bektesh, S., Collins, F.GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons.Science 260: 1130-1132, 1993. 3. Schindelhauer, D., Schuffenhauer, S., Gasser, T., Steinkasserer, A., Meitinger, T.The gene coding for glial cell line derived neurotrophic factor (GDNF) maps to chromosome 5p12-p13.1.Genomics 28: 605-607, 1995.
