ELISA's application
For quantitative measurement of human NRCAM in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
Gene tested
human NRCAM
ELISA standard curve
Concentration(pg/ml) + 0 + 62.5 + 125 + 250 + 500 + 1000 + 2000 + 4000 O.D. + 0.058 + 0.132 + 0.210 + 0.338 + 0.604 + 1.027 + 1.531 + 2.037
ELISA kit detection limits
62.5pg/ml to 4000pg/ml
ELISA's sensitivity
< 10pg/ml
Target of this ELISA
Natural and recombinant human NRCAM
Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);675;1308;2147;694;1382;2503 ELISA standard deviations 35.10;79.78;124.52;40.25;89.83;155.18 CV(%);5.2;6.1;5.8;5.8;6.5;6.2
ELISA kit description
GENTAUR's human NRCAM ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for NRCAM has been precoated onto 96-well plates. Standards (NSO, L30-N600) and your samples are measured with a biotin conjugated polyclonal from goat specific for NRCAM will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human NRCAM in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human NRCAM antibodies;1 ST0000-10;Lyophilized recombinant human NRCAM standard;10ng/tube×2 AR1107;Biotinylated anti- human NRCAM antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Anti-coagulation
heparin or EDTA
How the elisa works
Sample the amount of 100ul/well of the standard solution at 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human NRCAM standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each human NRCAM standard solution and each sample be measured in duplicate.
Minutes of TMB incubation
25-30
More gene info
Neuronal cell adhesion molecule is a protein that in humans is encoded by the NRCAM gene. Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. Also, this gene is expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antigen
NSO, L30-N600
Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Uniprot
Neuronal cell adhesion molecule
Gene name
neuronal cell adhesion molecule
Alternative
Bravo;hBravo;Neuronal cell adhesion molecule;Neuronal surface protein Bravo;Ng CAM related;Ng-CAM-related;NgCAM related cell adhesion molecule;NgCAM-related cell adhesion molecule;Nr CAM;Nr-CAM;Nrcam;NRCAM_HUMAN
Gen protein identity
Q92823
Entrez nr.
4897
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED