ELISA's application
For quantitative measurement of rat Neuropilin-2 in cell culture supernatant, cell lysate and tissue homogenates.
Gene tested
rat Neuropilin-2
ELISA standard curve
Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.011 + 0.124 + 0.269 + 0.516 + 0.917 + 1.401 + 1.811 + 1.995
ELISA kit detection limits
156pg/ml to 10000pg/ml
ELISA's sensitivity
< 10pg/ml
Target of this ELISA
Natural and recombinant rat Neuropilin-2
Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);1.6;3.5;6.1;1.9;4.2;7.3 ELISA standard deviations 0.058;0.161;0.317;0.089;0.231;0.467 CV(%);3.6;4.6;5.2;4.7;5.5;6.4
ELISA kit description
GENTAUR's rat Neuropilin-2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibodies from mouse specific for Neuropilin-2 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, Q23-D857) and your samples are measured with a biotin conjugated polyclonal from goat specific for Neuropilin-2 is added subsequently and then followed by washing with PBS or TBS buffers. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffers. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Neuropilin-2 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- rat Neuropilin-2 antibodies;1 ST0000-10;Lyophilized recombinant rat Neuropilin-2 standard;10ng/tube×2 AR1107;Biotinylated anti- rat Neuropilin-2 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Anti-coagulation
/
How the elisa works
Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml rat Neuropilin-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatant, cell lysate or tissue homogenates to the ELISA plate. Keep in mind that each rat Neuropilin-2 standard solution and each sample be measured in duplicate.
Minutes of TMB incubation
20-25
More gene info
NRP2(Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.
Antigen
NSO, Q23-D857
Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Uniprot
Neuropilin-2
Gene name
neuropilin 2
Alternative
MGC126574;Neuropilin 2;Neuropilin-2;Neuropilin2;NP 2;NP2;NPN 2;NPN2;NRP 2;Nrp2;NRP2_HUMAN;PRO2714;Receptor for VEGF165 and semaphorins class3;Vascular endothelial cell growth factor 165 receptor 2;Vascular endothelial growth factor 165 receptor 2;VEGF1265R2;VEGF165R2
Gen protein identity
O35276
Entrez nr.
81527
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus