ELISA's application
For quantitative measurement of mouse Neuropilin-2 in cell culture supernatant, cell lysate and tissue homogenates.
Gene tested
murine Neuropilin-2
ELISA standard curve
Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.004 + 0.047 + 0.106 + 0.200 + 0.403 + 0.729 + 1.408 + 2.263
ELISA kit detection limits
156pg/ml to 10000pg/ml
ELISA's sensitivity
< 10pg/ml
Target of this ELISA
Natural and recombinant mouse Neuropilin-2
Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);1590;3425;6308;1736;3621;6738 ELISA standard deviations 68.37;178.1;359.6;95.48;231.7;464.9 CV(%);4.3;5.2;5.7;5.5;6.4;6.9
ELISA kit description
GENTAUR's mouse Neuropilin-2 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for Neuropilin-2 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, Q23-D863) and your samples are measured with a biotin conjugated polyclonal from goat specific for Neuropilin-2 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse Neuropilin-2 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodies
A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse Neuropilin-2 antibodies;1 ST0000-10;Lyophilized recombinant mouse Neuropilin-2 standard;10ng/tube×2 AR1107;Biotinylated anti- mouse Neuropilin-2 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Anti-coagulation
/
How the elisa works
Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse Neuropilin-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, cell lysate or tissue homogenates to the ELISA plate. Keep in mind that each mouse Neuropilin-2 standard solution and each sample be measured in duplicate.
Minutes of TMB incubation
25-30
More gene info
NRP2(Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.
Antigen
NSO, Q23-D863
Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Uniprot
Neuropilin-2
Gene name
neuropilin 2
Alternative
MGC126574;Neuropilin 2;Neuropilin-2;Neuropilin2;NP 2;NP2;NPN 2;NPN2;NRP 2;Nrp2;NRP2_HUMAN;PRO2714;Receptor for VEGF165 and semaphorins class3;Vascular endothelial cell growth factor 165 receptor 2;Vascular endothelial growth factor 165 receptor 2;VEGF1265R2;VEGF165R2
Gen protein identity
O35375
Entrez nr.
18187
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus