ELISA's application
For quantitative measurement of rat Neuropilin-1 incell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
Gene tested
rat Neuropilin-1
ELISA standard curve
Concentration(ng/ml) + 0 + 0.78 + 1.56 + 3.12 + 6.25 + 12.5 + 25 + 50 O.D. + 0.025 + 0.065 + 0.119 + 0.210 + 0.369 + 0.749 + 1.355 + 2.007
ELISA kit detection limits
780pg/ml to 50000pg/ml
ELISA's sensitivity
< 20pg/ml
Target of this ELISA
Natural and recombinant rat Neuropilin-1
Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);8.3;21;30;8.6;24;35 ELISA standard deviations 0.315;0.987;1.62;0.421;1.32;2.38 CV(%);3.8;4.7;5.4;4.9;5.5;6.8
ELISA kit description
GENTAUR's rat Neuropilin-1 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for Neuropilin-1 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, F22-D854) and your samples are measured with a biotin conjugated polyclonal from goat specific for Neuropilin-1 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more rat Neuropilin-1 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- rat Neuropilin-1 antibodies;1 ST0000-50;Lyophilized recombinant rat Neuropilin-1 standard;50ng/tube×2 AR1107;Biotinylated anti- rat Neuropilin-1 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Anti-coagulation
heparin or EDTA
How the elisa works
Sample the amount of 100ul/well of the standard solution at 50ng/ml, 25ng/ml, 12.5ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml, 0.78ng/ml rat Neuropilin-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatant, serum or plasma(heparin, EDTA) to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each rat Neuropilin-1 standard solution and each sample is measured in duplicate.
Minutes of TMB incubation
15-20
More gene info
NRP1(Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.
Antigen
NSO, F22-D854
Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Uniprot
Neuropilin-1
Gene name
neuropilin 1
Alternative
A5 protein;BDCA4;BLOOD DENDRITIC CELL ANTIGEN 4;CD 304;CD304;DKFZp686A03134;DKFZp781F1414;Neuropilin-1;Neuropilin1;Neuropilin1 transmembrane receptor;NP1;NPN1;NRP 1;NRP;NRP1;NRP1_HUMAN;Vascular endothelial cell growth factor 165 receptor;VEGF165R
Gen protein identity
Q9QWJ9
Entrez nr.
246331
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus