ELISA's application
For quantitative measurement of rat Neurotrophin-3 in cell culture supernatant and serum.
Gene tested
Rat Neurotrophin-3
ELISA standard curve
Concentration(pg/ml) + 0 + 15.6 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 O.D. + 0.043 + 0.102 + 0.161 + 0.259 + 0.496 + 0.827 + 1.476 + 2.364
ELISA kit detection limits
15.6pg/ml to 1000pg/ml
ELISA's sensitivity
< 2pg/ml
Target of this ELISA
Natural and recombinant rat Neurotrophin-3
Cross reaction
There is cross-reactivates with NT-4
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);94;382;624;123;417;716 ELISA standard deviations 4.42;20.63;37.44;6.4;24.2;50.84 CV(%);4.7;5.4;6;5.2;5.8;7.1
ELISA kit description
GENTAUR's rat Neurotrophin-3 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for Neurotrophin-3 was used as capure antibodies on the ELISA test plate. The internal standards (sf21, Y139-T257) and your samples are measured with a biotin conjugated polyclonal from goat specific for Neurotrophin-3 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more rat Neurotrophin-3 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- rat Neurotrophin-3 antibodies;1 ST0000-10;Lyophilized recombinant rat Neurotrophin-3 standard;10ng/tubex2 AR1107;Biotinylated anti- rat Neurotrophin-3 antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Anti-coagulation
/
How the elisa works
Sample the amount of 100ul/well of the standard solution at 1000 pg per ml, 500, 25, 125, 62.5, 31.25, 15.6 pg/ml rat Neurotrophin-3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatants or serum to the ELISA plate. Keep in mind that each rat Neurotrophin-3 standard solution and each sample be measured in duplicate.
Minutes of TMB incubation
20-25
More gene info
Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.
Antigen
sf21, Y139-T257
Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Uniprot
Neurotrophin-3
Gene name
neurotrophin 3
Alternative
AI316846;_x000D_AI835689;_x000D_HDNF;_x000D__x000D__x000D_MGC129711;_x000D_Nerve growth factor 2;_x000D_Neurotrophic factor;_x000D_Neurotrophin 3 (HDNF/NT 3);_x000D_Neurotrophin 3 precursor;_x000D_Neurotrophin3;_x000D_NGF 2;_x000D_NGF2;_x000D_NT3;_x000D_NTF 3;_x000D_NTF3
Gen protein identity
P18280
Entrez nr.
81737
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus