ELISA's application
For quantitative measurement of rat CNTF in cell culture supernatant, serum and plasma heparin, EDTA, citrate .
Gene tested
Rat CNTF
ELISA standard curve
Concentration(pg/ml) + 0 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 + 2000 O.D. + 0.024 + 0.049 + 0.076 + 0.123 + 0.268 + 0.563 + 1.338 + 2.294
ELISA kit detection limits
31.2pg/ml to 2000pg/ml
ELISA's sensitivity
< 10pg/ml
Target of this ELISA
Natural and recombinant rat CNTF
Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);107;452;1287;193;643;1057 ELISA standard deviations 4.8;18.9;50.2;16.2;52.7;77.2 CV(%);4.5;4.2;3.9;8.4;8.2;7.3
ELISA kit description
GENTAUR's rat CNTF ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for CNTF was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, A2-M200) and your samples are measured with a biotin conjugated polyclonal from goat specific for CNTF will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more rat CNTF in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- rat CNTF antibodies;1 ST0000-10;Lyophilized recombinant rat CNTF standard;10ng/tube×2 AR1107;Biotinylated anti- rat CNTF antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Anti-coagulation
heparin, EDTA or citrate
How the elisa works
Sample the amount of 100ul/well of the standard solution at 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml rat CNTF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatant, serum or plasma( heparin, EDTA, citrate) to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each rat CNTF standard solution and each sample is measured in duplicate.
Minutes of TMB incubation
25-30
More gene info
Ciliary neurotrophic factor(CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid(aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
Antigen
E.coli, A2-M200
Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Uniprot
Ciliary neurotrophic factor
Gene name
ciliary neurotrophic factor
Alternative
AI429687;Ciliary Neurotrophic Factor;HCNTF;MGC41235;OTTHUMP00000174731;OTTMUSP00000029848
Gen protein identity
P20294
Entrez nr.
25707
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus