ELISA's application
For quantitative measurement of mouse BDNF in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA, citrate .
Gene tested
murine BDNF
ELISA standard curve
Concentration(pg/ml) + 0 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 + 2000 O.D. + 0.016 + 0.051 + 0.082 + 0.161 + 0.337 + 0.834 + 1.668 + 2.444
ELISA kit detection limits
31.2pg/ml to 2000pg/ml
ELISA's sensitivity
< 2pg/ml
Target of this ELISA
Natural and recombinant mouse BDNF
Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);105;436;1217;136;524;1279 ELISA standard deviations 3.57;16.13;49.90;9.24;38.25;94.65 CV(%);3.4;3.7;4.1;6.8;7.3;7.4
ELISA kit description
GENTAUR's mouse BDNF ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for BDNF was used as capure antibodies on the ELISA test plate. The internal standards (sf21, H129-R247) and your samples are measured with a biotin conjugated polyclonal from goat specific for BDNF will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse BDNF in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodies
A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse BDNF antibodies;1 ST0000-10;Lyophilized recombinant mouse BDNF standard;10ng/tube×2 AR1107;Biotinylated anti- mouse BDNF antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Anti-coagulation
heparin, EDTA or citrate
How the elisa works
Sample the amount of 100ul/well of the standard solution at 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse BDNF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(heparin, EDTA, citrate) to the ELISA plate. Keep in mind that each mouse BDNF standard solution and each sample be measured in duplicate.
Minutes of TMB incubation
20-25
More gene info
Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.
Antigen
sf21, H129-R247
Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Uniprot
Brain-derived neurotrophic factor
Gene name
brain-derived neurotrophic factor
Alternative
Abrineurin;ANON2;Brain derived neurotrophic factor;BULN2;MGC34632;Neurotrophin
Gen protein identity
P21237
Entrez nr.
12064
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus