ELISA's application
For quantitative measurement of rat BDNF in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA, citrate .
Gene tested
Rat BDNF
ELISA standard curve
Concentration(pg/ml) + 0 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 + 2000 O.D. + 0.016 + 0.051 + 0.082 + 0.161 + 0.337 + 0.834 + 1.668 + 2.444
ELISA kit detection limits
31.2pg/ml to 2000pg/ml
ELISA's sensitivity
< 2pg/ml
Target of this ELISA
Natural and recombinant rat BDNF
Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);109;447;1195;121;468;1257 ELISA standard deviations 3.60;17.43;51.39;8.35;33.23;90.5 CV(%);3.3;3.9;4.3;6.9;7.1;7.2
ELISA kit description
GENTAUR's rat BDNF ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for BDNF was used as capure antibodies on the ELISA test plate. The internal standards (sf21, H129-R247) and your samples are measured with a biotin conjugated polyclonal from goat specific for BDNF will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more rat BDNF in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- rat BDNF antibodies;1 ST0000-10;Lyophilized recombinant rat BDNF standard;10ng/tube×2 AR1107;Biotinylated anti- rat BDNF antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Anti-coagulation
heparin, EDTA or citrate
How the elisa works
Sample the amount of 100ul/well of the standard solution at 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml rat BDNF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatant, serum or plasma(heparin, EDTA, citrate) to the ELISA plate. Keep in mind that each rat BDNF standard solution and each sample be measured in duplicate.
Minutes of TMB incubation
20-25
More gene info
Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.
Antigen
sf21, H129-R247
Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Uniprot
Brain-derived neurotrophic factor
Gene name
brain-derived neurotrophic factor
Alternative
Abrineurin;ANON2;Brain derived neurotrophic factor;BULN2;MGC34632;Neurotrophin
Gen protein identity
P23363
Entrez nr.
24225
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus