Category
Primary Antibodies
Long description
Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFR , which is a cell surface receptor for GDNF1,2. The cDNAs encoding GDNFR from human, rat, chicken and mouse have been cloned recently1-5. GDNFR was also termed Ret ligand 1 (RETL1) or TGF- -related neurotrophic factor receptor 1 (TrnR1) and nominated as GFR -1 recently5-7. GFR -1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFR and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.
Antibody come from
Synthetic peptide corresponding to amino acids 369 to 382 of human GFR -1
Other description
Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative. DEAE Purification
Clone
Polyclonal
Antigen-antibody binding interaction
Rabbit anti GFRa-1 (IN ) (GDNFRa) (GDNF receptor) Antibody
Antibody is raised in
Rabbit
Antibody's reacts with
Human, Mouse, Rat
Antibody's reacts with these species
This antibody doesn't cross react with other species
Antibody's specificity
No Data Available
Application
Western Blot, IHC
Antibody's suited for
Detects human GFR -1 by Western blot at 1 µg/ml. Optimal concentration should be evaluated by serial dilutions.
Storage
-20ºC
Relevant references
1. Jing, S., et al. 'GDNF-induced activation of the Ret protein tyrosien kinase is mediated by GDNFR- , a novel receptor for GDNF.' Cell, 1996, 85, 1113-1124._x000B__x000B_2. Treanor, J.J.S., et al. 'Characterization of a multicomponent receptor for GDNF.' Nature 1996, 382, 80-83._x000B_ _x000B_3. Sanicola, M., et al. 'Glial cell line-derived neurotrophic factor-dependent RET activation can be mediated by two different cell-surface accessory proteins.' Proc. Natl. Acad. Sci. USA 1997, 94, 6238-6243 _x000B__x000B_4. Buj-Bello, A., et al. 'Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase.' Nature 1997, 387, 721-724 _x000B__x000B_5. Dey, B.K., et al. 'Cloning of a novel murine isoform of the glial cell line-derived neurotrophic factor receptor.' Neuroreport 1998, 9, 37-42_x000B__x000B_6. Baloh RH, et al. 'TrnR2, a novel receptor that mediates neurturin and GDNF signaling through Ret.' Neuron 1997, 18, 793-802_x000B__x000B_7. Nomenclature Committee. Nomenclature of GPI-linked receptors for the GDNF ligand family. GFR .. Neuron 1997, 19, 485 _x000B_
Protein number
see ncbi
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name
Oryctolagus cuniculus