Category
Primary Antibodies
Long description
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the Mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.
Antibody come from
RNF404 is a Mouse monoclonal IgG2a antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a neurofilament preparation of calf brain tissue.
Other description
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Clone
RNF404
Antigen-antibody binding interaction
Mouse anti Neurofilament 200 kD Antibody
Antibody is raised in
Mouse
Antibody's reacts with
Bovine,Canine,Chicken,Guinea Pig,Human,Monkey,Mouse,Rabbit,Rat,Sheep,Xenopus
Antibody's reacts with these species
This antibody doesn't cross react with other species
Antibody's specificity
RNF404 reacts exclusively with the phosphorylated isoform of the 200 kD neurofilament protein.
Research interest
Cytoskeleton,Neurobiology,Stem cell research
Application
Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
Antibody's suited for
RNF404 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:50 – 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:500 for immunoblotting applications.
Storage
Store at 4°C, or in small aliquots at -20°C.
Relevant references
1. No references available
Protein number
see ncbi
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus