Category
Primary Antibodies
Long description
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. CNTF is a polypeptide hormone and nerve growth factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations including astrocytes. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor. CNTF has also been shown to be expressed by cells on the bone surface, and to reduce the activity of bone forming cells, osteoblasts.
Antibody come from
Human recombinant ciliary neurotrophic factor (hCNTF).
Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
Clone
Polyclonal
Antigen-antibody binding interaction
Rabbit anti Ciliary Neurotropic Factor (CNTF) antibody conjugated to Biotin Antibody
Antibody is raised in
Rabbit
Antibody's reacts with
Human
Antibody's reacts with these species
This antibody doesn't cross react with other species
Antibody's specificity
No Data Available
Application
Enzyme Immunoassay
Antibody's suited for
Applications: ELISA: This antibody has been used as a detection antibody at 2 µg/ml in a two site ELISA with AS23. Typically the ELISA has a working range of 0-20,000 pg/ml and a sensitivity of 10 pg/ml when used with unconjugated capture antibody (Cat. No. L100M). Recommended sample volumes are 25-200 µl. Recovery of hCNTF in human plasma is typically > 85% in the ELISA. (Kenney, JS, Masada, MP, and Allison AC, Laboratory Methods in Immunology, Vol. I,231, 1990).
Storage
4ºC
Relevant references
1. Lam, A., et al. 'Sequence and structural organization of the human gene encoding ciliary neurotrophic factor.' Gene, 1991, 102, 271-276_x000B__x000B_2. Cargill, M., et al. 'Characterization of single-nucleotide polymorphisms in coding regions of human genes.' Nat Genet 1999, 22, 231-238
Protein number
see ncbi
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name
Oryctolagus cuniculus
French translation
anticorps