Synonyms
CNTFR, CNTF R alpha
Alternates names
CNTFR, CNTF R alpha
Taglines
This receptor and its cognate ligand support the survival of neurons.
Biomolecule/Target
CNTFR/CNTFR-alpha
NCBI Gene ID #.
1271
NCBI Gene Symbol
CNTFR
Gene Source
Human
Accession #
P26992
Recombinant
Yes
Source
HEK 293 cells
Purity
N/A
Endotoxin Level
< 1.0 EU per μg of the rh CNTFR alpha by the LAL method.
Activity (Specifications/test method)
Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 μg/ml (100 μl/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 μg/ml (100 μl/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.
Results
Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 μg/ml (100 μl/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.
Molecular Weight
This protein rh CNTFR alpha is fused with polyhistidine tag at the C-terminus, has a calculated MW of 36.9 kDa. The predicted N-terminus is Gln 23. DTT-reduced Protein migrates as 45-48 kDa due to glycosylation.
Storage Temp.
-20°C
Shipping
gel pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Description
The CellExpCNTFR/CNTFR-alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants