Synonyms
KLK8, Kallikrein-8, Neuropsin, Ovasin, NRPN, PRSS19, TADG14, NP, hK8
Alternates names
KLK8, Kallikrein-8, Neuropsin, Ovasin, NRPN, PRSS19, TADG14, NP, hK8
Taglines
≥95% pure, active, human cell expressed, human recombinant KLK-8 / Kallikrein-8 - serves as a marker of ovarian cancer.
Biomolecule/Target
KLK-8 / Kallikrein-8
NCBI Gene ID #.
11202
NCBI Gene Symbol
KLK8
Gene Source
Human
Accession #
O60259
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its ability to inhibit thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >500 pmoles / min / μg.
Results
>500 pmoles / min / μg
Molecular Weight
This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 25.8 kDa. The predicted N-terminus is Gln 29. DTT-reduced Protein migrates as 38 kDa in SDS-PAGE due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 8.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants