Synonyms
ERBB2, CD340, HER-2/neu, HER2, MLN19, NEU, NGL, TKR1
Alternates names
ERBB2, CD340, HER-2/neu, HER2, MLN19, NEU, NGL, TKR1
Taglines
Involved in the signal transduction pathways leading to cell growth and differentiation.
Biomolecule/Target
ErbB2
NCBI Gene ID #.
2064
NCBI Gene Symbol
ErbB2
Gene Source
Human
Accession #
P04626
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its ability to block anti-ErbB2 mediated inhibition of SK-BR-3 human breast cancer cell proliferation. The ED50 for this effect is typically 5-45 ng/ml in the presence of 0.6 μg/ml goat anti-hErbB2.
Results
Measured by its ability to block anti-ErbB2 mediated inhibition of SK-BR-3 human breast cancer cell proliferation. The ED50 for this effect is typically 5-45 ng/ml in the presence of 0.6 μg/ml goat anti-hErbB2.
Molecular Weight
This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 72.4 kDa. The predicted N-terminus is Thr 23. DTT-reduced Protein migrates as 90-110 kDa due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants