Western Blot: 1:2,000. Immunocytochemistry/Immunofluorescence: 1:1,000
Full length recombinant human DJ-1 expressed in and purified from E. coli
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Frozen (Polar Packs)
11315
Q99497
NA
ICC, WB, IF
Rabbit IgG
Whole serum
liquid
If you buy Antibodies supplied by Neuromics they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by Neuromics. Rabbit antibodies are very stable and can be stored for several days at room temperature. Neuromics adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
anticorps