About recombinant protein
Neuroserpin is an inhibitory serpin that is expressed predominantly in central nervous system. Although the physiological target of neuroserpin is still unclear, cumulative evidence suggest that it plays an important role in controlling proteolytic degradation of extracellular matrix (ECM) during synaptogenesis and the subsequent development of neuronal plasticity. In the adult brain, neuroserpin is secreted from the growth cones of neurons in areas where synaptic changes are associated with learning and memory, i.e. cerebral cortex, hippocampus, and amygdala. The neuroprotective role of neuroserpin has been demonstrated in transgenic mice lacking neuroserpin expression. The deficiency of neuroserpin in these mice was associated with motor neuron disease characterized by axonal degradation. In humans, defects in neuroserpin, caused by point mutations in the neuroserpin gene, underlie a hereditary disorder called the familial encephalopathy with neuroserpin inclusion bodies (FENIB). Recombinant human neuroserpin is a 44.6 kDa non-glycosylated protein containing 394 amino-acid residues.
Other name of the recombinant
PI12, neuroserpin, PI12, Neuroserpin, Peptidase inhibitor 12, PI-12
Recombinant in
E. coli
SEQUENCE
MTGATFPEEA IADLSVNMYN RLRATGEDEN ILFSPLSIAL AMGMMELGAQ GSTQKEIRHS MGYDSLKNGE EFSFLKEFSN MVTAKESQYV MKIANSLFVQ NGFHVNEEFL QMMKKYFNAA VNHVDFSQNV AVANYINKWV ENNTNNLVKD LVSPRDFDAA TYLALINAVY FKGNWKSQFR PENTRTFSFT KDDESEVQIP MMYQQGEFYY GEFSDGSNEA GGIYQVLEIP YEGDEISMML VLSRQEVPLA TLEPLVKAQL VEEWANSVKK QKVEVYLPRF TVEQEIDLKD VLKALGITEI FIKDANLTGL SDNKEIFLSK AIHKSFLEVN EEGSEAAAVS GMIAISRMAV LYPQVIVDHP FFFLIRNRRT GTILFMGRVM HPETMNTSGH DFEEL
Activity
Determined by the dose - dependent stimulation of the proliferation of rat C6 cells using a concentration range of 0.3 - 0.6 μg/mL.
Keep at
The lyophilized Neuroserpin recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Neuroserpin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Neuroserpin recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Rec. PROTEIN GI#
170295807
Accession nr
NP_001116224.1
Pureity
Greater than 96% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
About
This product is for research use only.
Protein's origin
Human
Vial
Lyophilized
NCBI ID
5274
Gene name
serpin peptidase inhibitor, clade I (neuroserpin), member 1
Source
Homo sapiens
Uniprot
Q99574
Additional source
Recombinants or rec. proteins
Group
recombinants