About recombinant protein
Neuropoietin is a newly identified member of the IL-6 cytokine family. Members of this family, including IL-6, IL-11, oncostatin M, leukemia inhibitory factor (LIF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine, and CNTF, display a four-helix bundle structure, and signal through gp130-containing receptor complexes. Neuropoietin, which is predominantly expressed in neuroepithelia during embryonic life, acts through a receptor complex comprising CNTF receptor-a component, gp 130, and LIF receptor. Like CNTF, it promotes the survival of embryonic motor neurons and could increase the proliferation of neural precursor cells in the presence of EGF and FGF-2. Interestingly, the human Neuropoietin gene has evolved toward a pseudogene, suggesting that the alternative signaling via CNTF is an effective compensatory pathway. Recombinant murine Neuropoietin is a 19.8 kDa protein containing 183 amino acid residues.
Other name of the recombinant
NP, Gm494, Cardiotrophin-2, Neuropoietin, CT-2
Recombinant in
E. coli
SEQUENCE
MAPISPSEPI GQAYSLALYM QKNTSALLQT YLQHQGSPFS DPGFSAPELQ LSTLPSAAVS FKTWHAMEDA ERLSRAQGAF LALTQHLQLV GDDQSYLNPG SPILLAQLGA ARLRAQGLLG NMAAIMTALG LPIPPEEDTL GFVPFGASAF ERKCRGYIVT REYGHWTDRA VRDLALLKAK YSA
Activity
The ED50 was determined by the dose - dependent stimulation of the proliferation of human TF - 1 cells is 0.5 - 0.8 μg/mL.
Keep at
The lyophilized Neuropoietin recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Neuropoietin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Neuropoietin recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Rec. PROTEIN GI#
38524598
Accession nr
NP_942155.1
Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
About
This product is for research use only.
Protein's origin
Murine
Vial
Lyophilized
NCBI ID
244218
Gene name
cardiotrophin 2
Source
Mus musculus
Uniprot
P83714
Additional source
Recombinants or rec. proteins
Group
recombinants