CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity. Recombinant human CNTF is synthesized as a 199 amino acid polypeptide (22.7 kDa) lacking a hydrophobic N-terminal signal for secretion.
The ED50 was determined by the dose - dependent proliferation of human TF - 1 cells is < 2.0 ng/mL, corresponding to a specific activity of > 5 x 105 units/mg.
Keep at
The lyophilized CNTF recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted CNTF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing CNTF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Rec. PROTEIN GI#
4758020
Accession nr
NP_000605.1
Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
