BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0 kDa homodimer of two 119 amino acid subunits linked by strong non-covalent interactions. Human and Mouse BDNF sequences are identical.
Biological activity was determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5 - 1.0 μg/mL.
Keep at
The lyophilized BDNF recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted BDNF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing BDNF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Rec. PROTEIN GI#
219842288
Accession nr
NP_001137277.1
Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
