Anti-hGDNF specific antibody was purified by affinity chromatography and then biotinylated.
Ab raised in
Rabbit
Type
Polyclonal
Antigen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGDNF (human GDNF).
Immuno application
ELISA, Western Blot
Antibody advice
ELISA:To detect hGDNF by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 ug/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hGDNF. Sandwich: To detect hGDNF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human GDNF (XP-5143) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGDNF.Western Blot:To detect hGDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Keep at
GDNF antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
Works with
Human
Omim nr
729567
Ncbi nr
P39905
Vial with antibody
Lyophilized
NCBI GENE nr
2668
GENE symbol
GDNF
Antigene name
glial cell derived neurotrophic factor
Source
Homo sapiens
Protein nr
P39905
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
